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(PRSV-YK-resistant Strains) for Seeds and Leaves. I. Points to The seeds to be tested shall be intact, not crushed, and contain no foreign matter. After A and 20 μL of Proteinase K, mix using a vortex mixer for 30 seconds while avoiding Eppendorf tube, add 200 μL of GB3 buffer solution and 200 μL of ethanol (),.
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crushing leaf in eppendorf using vortex . Patent US9078455 Process for manufacturing tea . Gently mix the methanol-water and tea material on a vortex Grind the tea leaf to a fine powder using a pestle Decant into eppendorf tubes and centrifuge in Inquire Now; Crushing Leaf In Eppendorf Using Vortex Grinding .
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The crushing results were then transferred into a 1.5 ml eppendorf tube, plus 0.5 ml of CTAB extraction buffer (1.4 M NaCl, 2% CTAB, 50 mM EDTA, 1 M Tris-HCl pH 8.0 and 0, 2% -mercaptoetanol). The process of lysis of the cell wall is done by incubating the tube containing the leaf sample into the temperature waterbath of 65oC for 60 minutes.
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crushing leaf in eppendorf using vortex . Patent US9078455 Process for manufacturing tea . Gently mix the methanol-water and tea material on a vortex Grind the tea leaf to a fine powder using a pestle Decant into eppendorf tubes and centrifuge in Inquire Now; Crushing Leaf In Eppendorf Using Vortex Grinding .
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